Easily releasable myofilaments and myofibrillar protein turnover

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Properties of easily releasable myofilaments: are they the first step in myofibrillar protein turnover?

Myofibrillar proteins must be removed from the myofibril before they can be turned over metabolically in functioning muscle cells. It is uncertain how this removal is accomplished without disruption of the contractile function of the myofibril. It has been proposed that the calpains could remove the outer layer of filaments from myofibrils as a first step in myofibrillar protein turnover. Sever...

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Easily releasable myofilaments from skeletal and cardiac muscles maintained in vitro. Role in myofibrillar assembly and turnover.

Gentle treatment with an ATP-containing relaxing solution of isolated myofibrils from rat diaphragm, soleus, extensor digitorum longus, and left atria maintained in vitro releases a small amount of myofilaments constituting less than 5% of total myofibrillar protein. Successive extraction of myofibrils produced little further filament release. Releasable myofilaments lack alpha-actinin (Mr = 95...

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Effect of starvation or treatment with corticosterone on the amount of easily releasable myofilaments in rat skeletal muscles.

Treatment of isolated myofibrils with an ATP-containing relaxing solution results in the dissociation of a preformed quantity of myofilaments called 'easily releasable myofilaments'. Van der Westhuyzen, Matsumoto & Etlinger [(1981) J. Biol. Chem. 256, 11791-11797] presented experimental evidence that these myofilaments represent intermediate products in the turnover of myofibrillar proteins. To...

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The rates of gain, catabolism, synthesis and reutilization of myofibrillar protein were measured in adult rats fed a protein-free diet, low protein diet (2% lactalbumin) or control diet (10% lactalbumin) for 14 to 31 days. Two forms of synthesis were measured: exogenous synthesis (nitrogen derived from diet) and endogenous synthesis (nitrogen derived from catabolized body protein). The rate of ...

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ژورنال

عنوان ژورنال: The FASEB Journal

سال: 2006

ISSN: 0892-6638,1530-6860

DOI: 10.1096/fasebj.20.4.a50-c